Agar

Agar is a gel-like medium made from seaweed that is used to grow and propagate fungal cultures. It is often used to isolate and grow a pure culture of a specific mushroom strain. A small piece of mycelium from the desired strain is placed onto the agar, where it can grow and spread out over the surface of the medium.

Liquid culture is another technique used for cloning mushrooms, in which mycelium is grown in a liquid solution. This method involves placing a small piece of mycelium into a sterile liquid solution (such as water or nutrient broth), which is then agitated or stirred to help the mycelium disperse throughout the liquid. The resulting liquid culture can then be used to inoculate larger batches of substrate for mushroom cultivation.

Grain spawn, agar, and liquid culture are all techniques used for cloning mushrooms. Grain spawn is a highly colonized substrate that can be used to inoculate larger batches of substrate, and liquid culture is a technique used for growing mycelium in a liquid solution. These methods can help ensure that desired traits are preserved and passed on to future generations of mushrooms